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Granulocytes from HR, eRA and laRA displayed a normal level of spontaneous (figure 5A) and stimulated (figure 5B) generation of ROS; however, after stimulation with opsonised zymosan the time needed to reach peak ROS production was significantly prolonged in HR, eRA and laRA (figure 5C).
Less than 1% of the cells treated with Cytochalasin B invaded irrespective of whether they were mechanically stimulated (Figure 5C).
In MBP-2Apro-treated extracts, translation of (E/−) or (E/+) luc mRNAs was strongly stimulated (Figure 5B, grey bars).
While non-tolerant control T cells of Agneg mice secreted IL-2 upon peptide stimulation, no IL-2 was detectable when the T cells of Agpos mice were stimulated (Figure 2B).
When the function a = f(T0) is represented for the 3 part of the body stimulated (figure 8C) to delineate the domains for obtaining responses elicited by Aδ- or C-fiber, one notices that aAC is always the weaker when the foot is stimulated and the larger when the forehead is stimulated.
For each cytokine studied expression levels relative to the housekeeping gene HPRT were determined for both CS stimulated (Figure 1) and unstimulated PBMCs (Figure 2), and the fold change in expression level in the CS-stimulated cells compared to the unstimulated cells at each timepoint determined (Table 1).
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Interestingly, the mean CD86 level is significantly higher in APC with high stimulation activity than APC with low stimulation activity in both LPS stimulated cells (Figure 5d, p = 0.0009) and polyI C stimulated cells (Figure 5e, p = 0.01).
After 24 hours of stimulation, we found the cells invaded just as well as they did when they were seeded on top of the matrices prior to stimulation (6% invasion in non-stimulated and 13% in stimulated cultures) (Figure 4B).
YVADcmk completely abrogated apoptosis induced by LPS stimulated monocytes (Figure 1).
ASC, similar to caspase-1, was identified both in the supernatants and microvesicles from LPS stimulated monocytes (Figure 2C).
Results demonstrated a marked increase in relative expression of Ncf1 but not Ncf2 or gp91phox in stimulated cells (Figure 6).
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