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No cytokines were produced to a control antigen or when cells were not stimulated (data not shown).
Nineteen other signalling pathways present on the preliminary microarray experiments, and confirmed by real-time PCR, were not stimulated (data not shown).
Stimulated data were subtracted from unstimulated data to yield a reflex trace.
In these cell lines, tyrosine phosphorylation of some other cellular proteins is also stimulated (data not shown).
For both control and NPY stimulated data sets measurements of D and particle concentration did not show time dependence over the room temperature recording period (Supplementary Fig. 2) and were therefore pooled.
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The two methods are 'participatory diagramming', where the respondent creates a diagram that assists in their communication of answers, and 'graphic elicitation', where a researcher-prepared diagram is used to stimulate data collection.
Recent interest from data users on applications that utilize the uncertainties of evaluated nuclear reaction data has stimulated the data evaluation community to focus on producing covariance data to a far greater extent than ever before.
DREME finds 19 significant motifs in the unstimulated cell data, and only 11 in the stimulated cell data (E < 0.05).
Similar results for technical variation were found in the stimulated specimens (data not shown).
IDO mRNA could not be detected in unstimulated or stimulated MSCs (data not shown).
Control experiments were performed which demonstrated no effect of SB203580, PD098059 on the non-VEGF stimulated HIMEC (data not shown).
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