According to previous reports, high shear stress environment may be stimulated by the addition of glass beads in shake flask (Dobson et al. 2008).
Both native and recombinant enzyme were stimulated by the addition of divalent cations predominantly Mn2+, and strongly inhibited by bestatin and cysteine.
For STP-spiked sludge at test start (day 11), the comparison of both controls showed that microbial biomass was stimulated by the addition of sewage sludge, but there was no influence by NM-300K or silver nitrate.
Microbial growth was stimulated by the addition of C (Figure2c) and more so by the addition of CN (Figure2d), apart from in 0_0 in which microbial growth did not respond to added C or CN.
We describe here the isolation and characterisation of an active single-ring form of the GroEL protein (SR-A92T), which has an exceptionally low ATPase activity that is strongly stimulated by the addition of GroES.
Although the processes that can be used in order to achieve the desirable results vary, they still have the same principles; the use of microorganisms or their enzymes, that are either indigenous and are stimulated by the addition of nutrients or optimization of conditions, or are seeded into the soil.
The same effects were observed when calcium was stimulated by the addition of exogenous ATP.
As shown in Figure 6A, proton translocation driven by deamino-NADH in wild type membrane vesicles can be stimulated by the addition of 50 µM decylubiquinone.
Migration was stimulated by the addition of bone morphogenic protein-2 (BMP-2) (Sigma) into the media at a final concentration of 0.1 µg/mL.
T47D cell growth is stimulated by the addition of bivalent Anti-MUC1* and the characteristic bell-shaped growth curve is produced.
Cell proliferation analysis showed that the growth of both HMVEC-d and NHDF cells was stimulated by the addition of conditioned medium from WNT10A-overexpressing cells and was significantly inhibited by the addition of an anti-WNT10 antibody (Figures 4B and 4C).
