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After the initial incubation period and 24 hours in media without FCS to synchronize the cells, each sample was stimulated as per their conditions with DMEM/F12 supplemented with 5% FCS.
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7.5 × 10 RAW264.7 cells were seeded per well in 6-well plates and then stimulated as indicated.
To stimulate as much economic activity as the estimated cost of AB 233's tax expenditure, the new deduction would only have to prompt 4,141 new pet adoptions over five years -- or 828 more per year.
BV2 and shRNA expressing clones were plated at 104 per well and stimulated as above.
And mentally, as a rugby pro, I was not as stimulated as before.
Cells were stimulated as in (d).
T cells were seeded onto flat bottom polystyrene 48-well plates (Costar, Corning Inc). at 10 cells/ml in a volume of 500 μl per well and stimulated as described above.
T cells were resuspended in serum-free X-VIVO 15 medium supplemented with gentamycin (Lonza), seeded onto flat bottom polystyrene 48-well plates (Costar, Corning Inc). at 10 cells/ml in a volume of 1 ml per well and stimulated as described above.
I'm pretty stimulated as it is.
Mitochondria were stimulated as described earlier.
Cells were stimulated as described above.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com