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Db-derived macrophages showed more extreme polarisation towards an M1 phenotype when classically stimulated, as expected (Fig. 4A), and, consistent with early-wound-derived myeloid cells, also showed an elevated M2 polarisation response when stimulated with alternative-activation-inducing factors (Fig. 4B).
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Forskolin dose-dependently stimulated β-galactosidase activity in these cells, as expected.
EGF alone also stimulated STAT3 phosphorylation as expected.
As shown in Figure 5, in wild type primary murine lung endothelial cells bearing TSP1, the addition of DEA/NO increased intracellular cGMP production, while treatment with Aβ inhibited DEA/NO stimulated cGMP production, as expected.
Inducible expression of NFAT1 stimulates invasion as expected, and this was reduced to control levels in the presence of GPC6 shRNA.
Exogenous cystatin C did not stimulate nsph formation as expected, so this protein was not evaluated further.
Participants reported stimulating the beads as expected during the initial days after quitting, but most soon reduced the frequency of stimulation.
Another mutant Osx (1 288), which contains an intact activation domain but lacks a DNA-binding domain, was unable to stimulate reporter activity, as expected, and NO66 did not further reduce the activity of this mutant.
Employing an antibody that recognizes LKB1 phosphorylated at Ser we observed that treat-ment of LKB1+/+ wild-type MEFs with either forskolin to activate PKA or PMA to stimulate RSK, induced, as expected, a marked phosphorylation of LKB1 at Ser.
Human p53, as expected, stimulated the Hdm2 promoter, at 37°C.
Both mutants showed a reduction in Ins P6-stimulated ATPase activity, as expected since Arg and Lys are required for binding of Ins P6 [ 20].
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