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The conditioned media was used to stimulate serum-starved HB4a and C3.6 cells for 15 min.
Growth factors such as EGF or insulin were used to stimulate serum-starved ASPP2 and ASPP2 Δ3/Δ3) MEFs to induce RAS activation.
Env 2-3 serumlantibodies antobodies to HIV-1 envelope protein (gp120) as detected by Western blot in 39 of 43 subjects and to HIV-1 virus lysate by EIA in 28 of 43 subjects after three injections.
Primary outcome measure was stimulated serum C-peptide levels at 12 or 18 months.
We showed that KGF stimulated serum deprived Panc-1 cell proliferation as potently as did 10% FBS.
We stimulated serum starved wt, K1003R, Y1136F and Δ1245 cells with IGF-1 for different time points (0 20 min).
As primary outcome stimulated serum C-peptide levels were determined 90 min after a standardized liquid mixed meal.
Secondary analyses of the course of C-peptide secretion within groups found some preservation of fasting and stimulated serum C-peptide concentrations in the atorvastatin but not the placebo group.
Based on data on C-peptide levels from patient files of the German Diabetes Center it was calculated that for the primary endpoint 80 patients per group would allow to recognize a 20% difference between study groups in median stimulated serum C-peptide levels at 18 months with a type 1 error of <1%, power 95%.
CRH stimulated serum cortisol and plasma ACTH to less than 4%% of basal values.
administration of 1 μg corticotropin (synthetic ACTH1 24) for the measurement of stimulated serum cortisol concentrations.
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