Systemically, relaxin appears to directly stimulate expression of the ET-B receptor on endothelial cells, whereas stimulation of ET synthesis, resulting in increased engagement and activation of the endothelial ET-B receptor, appears to be the pathway in the kidney.
In transient transfection assays in XEN cells, we observed that activin could efficiently stimulate expression of this reporter, whereas p38 inhibition significantly reduced this stimulation, and inhibition of the Alk4/5/7 receptors with SB43154 completely abolished it.
In addition, qPCR test revealed that exogenously overexpressed SIRT1-WT is able to stimulate expression of TFEB downstream targets which are involved in the process of lysosomal biogenesis as well as lysosome-mediated degradative pathways (Fig. 5E), while SIRT1 knockdown decreases expression of these genes (Fig. S4H).
As a consequence, the ability of β subunits to stimulate expression of functional channels is underestimated.
IL-4 can regulate and stimulate expression of the MCP-1 by human endothelial cells [35].
We then directed our efforts to determine whether stress could stimulate expression of VdGARP1.
In contrast to PPARα agonists, the LXR agonist did not stimulate expression of enzymes involved in mitochondrial and peroxisomal oxidation.
Indeed, NGF is known to stimulate expression of the TF Egr-1 through a well-studied signaling pathway (Fig. 5A).
The mammalian SREBP-1a and SREBP-1c transcription factors stimulate expression of genes involved in fatty acid biosynthesis while SREBP-2 stimulates genes involved in cholesterol biosynthesis.
The ToxB protease was demonstrated to post-transcriptionally stimulate expression of LEE4 proteins and facilitate adhesion to epithelial cells [26], [27].
The use of CsA supported our previous data (Fig. 1D), showing that PMA is not acting through the calcineurin pathway to stimulate expression of RCAN1.4.
