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To stimulate degradation of misfolded collagen by autophagy, we utilized a low protein diet.
The results indicated that to correctly investigate performance of polymeric scaffolds in-vivo, all constituents that stimulate degradation such as correct pulsatile flow, pressure, and temperature should be implemented in-vitro to obtain accurate results.
The DAZL-deficient rat line was produced on a Sprague Dawley background and expresses a small hairpin RNA (shRNA) transgene designed to stimulate degradation of transcripts encoding DAZL [17].
We then hypothesized that if CB2-Rs are present in the brain, then antisense oligonucleotides complementary to CB2 mRNA transcript will block translation of or stimulate degradation of CB2 mRNA.
Using a computational approach in combination with in vivo and in vitro experiments, we previously showed that LhrA binds specifically to the 5'-end of the lmo0850 mRNA, encoding a small protein of unknown function, to repress translation and stimulate degradation of lmo0850 mRNA in an Hfq-dependent manner [23].
PA700 is the only proteasome activator, that is, known to stimulate degradation of protein substrates.
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TNF-alpha is shown as stimulating ECM, whereas in the model it stimulates degradation of ECM.
The degradation rate of large macromolecules was substantially higher (96% vs 81%) in ADMEC than control effluent, suggesting that MECs stimulated degradation of refractory organic matter and reduced humification.
Ornithine decarboxylase antizyme subfamily consists of three related antizyme members and ornithine decarboxylase antizyme-1 (OAZ) was first discovered and identified as an ornithine decarboxylase (ODC) inhibitory molecule by stimulating degradation of ODC protein [3], [4].
However, other possibilities, such as repressed synthesis or stimulated degradation, could not be fully ruled out.
These same properties, however, may be beneficial after disc herniation, stimulating degradation of herniated tissue and encouraging spontaneous disc resorption.
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