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TrxR1 inhibited by MQ was still functional as a pro-oxidant NADPH oxidase.
In rare instances, the protein fragment is still functional as bad codons can also be bypassed or edited at the level of mRNA by recoding mechanisms.
Collectively, this data indicate that the majority of the GFP-fused proteins are still functional as it was also found by Newman et. al [ 19].
It is however still functional as a single nucleotide polymorphism (SNP) in this site can cause skewing of XCI (Pugacheva et al. 2005).
As expected, these mutations abolished Jen1 ubiquitylation in response to glucose, but this construct was still functional, as judged by its ability to transport selenite, that can be used as a readout for Jen1 activity (McDermott et al., 2010).
It is therefore not clear whether an endosymbiont of an omnivore such as Blochmannia is still functional as a mutualist or rather a relic of a former mutualistic interaction.
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Deletion mutants of hrpZ are still functional and HrpV functions as a negative regulator of the T3SS [ 48- 50].
The joint evo-devo changes of these structures in mammals (where they are still functional, but with a new function), as well as in extant reptiles that have also evolved from the common ancestor with mammals, provide much evidence as to joint ontogenetic and phylogenetic transformation.
Focus was placed on constructs that (i) contained at least the minimal regions previously identified for the interaction with ORAI1 (Muik et al, 2009; Park et al, 2009; Yuan et al, 2009) and (ii) were still functional both for the coupling to as well as activation of ORAI1 channels.
It has no extraneous elements yet is still functional and easily recognizable as an individual branded object.
Irrespective of this fact, which is highly relevant to the nanomedicine of central nervous system, engineering the cationic character of defined protein stretches is revealed here as a promising and generic approach to promote the controlled oligomerization of biologically active protein species as still functional, regular nanoparticles.
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