The absence of stiffness dependent spreading and differentiation on PDMS, apparently contradictory to previous findings of cell differentiation dependence on substrate stiffness, can be explained by the ability of seeded cells to remodel the collagen layer present on viscoelastic PDMS, diminishing their sensitivity to substrate stiffness.
The model shows that TDB is only caused by thermal deformation, while TDSF consists of two parts caused by stiffness temperature dependence and thermal deformation, respectively.
The analytical model for the temperature drift of bias (TDB) and temperature drift of scale factor (TDSF) is established based on the analysis results of thermal deformation and stiffness temperature dependence.
Stem cell stiffness-dependent differentiation also appears similar to 2D cases, though tension dependence is due to integrin ligation rather than spreading [36].
The resulting data indicate that the bearing stiffness is dependent on the cooling distance and exhibits no significant dependence on the oscillation amplitude.
For fixed elastic stiffness, B, the dependence of v b on γ shows a simpler picture, see Fig. 4 c.
We investigate the stability of the fibre modes, their stiffness, and their dependence on the problem parameters, focusing on the influence of smoothing.
A dynamic analysis of the CVST system exposed to excitations of rail harmonic irregularities is carried out, pointing out the stiffness and damping dependence on the excitation frequency and the displacement amplitude.
In particular, fluorescence of pyrene-labeled PS (MPy-PS) films, with ∼1 mol% pyrene label, provides sensitivity to film stress relaxation and stiffness by the dependence of the ratio of the first to third vibronic peak intensities (I1/I3) of the pyrenyl dye to nanosecond-time-scale molecular caging.
In particular, our model predicts for the first time recently reported transitions from filopodial to "stick-slip" to gliding motility on ECMs of increasing stiffness, previously observed dependences of migration speed on ECM stiffness and ligand density, and high-resolution measurements of mechanosensitive protrusion dynamics during cell motility we newly obtained for this study.
