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Additionally, a stiffened conformation of Tm would have to relax fast enough to not limit the decay of Position M. Fourth, a grossly inhomogeneous myosin distribution along the thin filament is the greatest concern for our model of isometric contraction, but variance in bound myosin is also a concern.
The N-terminal segment of the luminal region of DC-LAMP is proline-rich and densely O-glycosylated (residues 28 to 221, Figure 1A), which implies an elongated, stiffened conformation due to steric restraints imposed by the glycan moieties [ 45].
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He visibly stiffened.
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