We thank Dr's Ludovico and Rebecca Cademartiri for testing of the methods of sterilization of patterned paper and Professor Emanuel Carrilho for assistance with patterning of paper.
Traditional surgeons were also provided with a tool box including surgical (scalpel) blades, scalpel handles, latex hand gloves, sterilization instruments, and paper towel rolls, and traditional nurses received also the tool box including latex hand gloves, sterilization instruments, and paper towel rolls.
Hydrogen peroxide (H2O2), an important oxidizing, bleaching, and sterilizing agent, is widely used in food processing, pulp and paper bleaching, sterilization, liquid-based fuel cells, and clinical applications [1 7].
The samples were blotted dry with filter paper; next, surface sterilization of shoot and root pieces was performed with the following immersion sequence: 70% ethanol for 1 min and 3% sodium hypochlorite for 5 min. Then, they were rinsed four times with sterile water and dried in laminar flow.
Sterilization of the wax-patterned paper was problematic because prolonged heating of patterned substrates above >100°C caused the wax to melt and to spread laterally across the paper blurring the patterns.
Teflon pans only require wiping with a paper towel and some sterilization. 1 capful of bleach will sterilize 5 gallons (20 L) of water - you don't need more than a tiny bit for one or two gallons (4 - 8 L).
Specimens were sterilized using plasma sterilization (H2O2).
After sterilization, cellulase enzyme, Spezyme CP (60 filter paper units per ml; Genencor, Palo Alto, USA), and Saccharomyces cerevisiae D5A were added to the flasks, followed by incubation at 35°C for 24 h while rotating at 170 rpm.
But not to worry, the process of making poo paper involves a series of sterilizations, washing, and processing until fibers are left over to create paper.
I only learned about that sterilization conversation years later, when I was writing a paper on sexual assault and women with disabilities for a graduate school class.
This paper describes the progress in terms of EO sterilization and concludes that it remains a promising field to explore and develop.
