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His Austrian team was developing space suit technology, while other crews tested three-dimensional cameras, radar, rover vehicles, communications and sterile testing systems.
Similar(59)
Then, with the help of a flame-sterilized platinum handle, the bacterial cells were transferred to a sterile test tube containing 0.85% NaCl solution until reaching an absorption between 0.08 and 0.10 in a spectrophotometer at the wavelength of 625 nm (corresponding to approximately 1 × 108 cells).
Two hundred milliliter of sterile NB was distributed in 20 sterile test tubes.
The suspension was transferred aseptically to 5 ml of sterile distilled water in sterile test tube.
Aliquots of 1,000 μL of the suspension containing about 105 cells mL−1 were transferred to sterile test tubes containing the samples.
After overnight incubation, all the three samples were centrifuged to separate yeast cells and supernatants were filtered using membrane filters to obtain clear sterile test samples.
One hundred and sixty milliliter of sterile (NB + 0.1% tryptophan) was prepared and distributed in 16 sterile test tubes with pH 5, 7, 9 and 11 (4 tubes each with same pH), respectively.
The hydroxyapatite discs and the bacterial suspension were added into sterile test tubes containing 2 ml of tryptic soy broth to make the final concentration of the bacterial cells approximately 2 × 107 CFU/ml.
The bark, leaves, fruit pulp and seed extracts of S. caryophyllatum dissolved in 5% dimethyl sulfoxide (DMSO) were first diluted to the highest concentration (1000 μg/mL) to be tested, and then serial twofold dilutions were made in order to obtain a concentration range from 62.5 1000 μg/mL in 10 mL sterile test tubes containing nutrient broth.
MG132 was first dissolved in a drop of 100% ethanol followed by DMEM/HAM F-12K medium without addition of FBS, and the stock solution was stored at 4°C. Cell culture was harvested and transferred into 6 sterile test tubes.
The collected swab samples were put into sterile test tube.
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