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The samples were collected in 500 ml sterile sampling bottles.
The first 3 4 streams of milk were discarded, and approximately 10 ml of milk was collected into sterile sampling bottles.
The roots were then transferred to a sterile sampling bag and squeezed with a soft-headed hammer in order to release root-associated/endophytic bacterial cells.
Sampling was done in 60-ml sterile sampling bottles and transported in a cool box at 4 °C to the laboratory in Isiolo County referral hospital for samples from Isiolo and University of Nairobi's Food microbiology laboratory for samples from Nairobi.
One water sampler, trained in sterile sampling techniques, worked with each survey team to collect water used for drinking by 12 selected households in each cluster.
Samples were then transferred to sterile sampling bags (VWR), and Triton X-100 (Sigma) was added to a final concentration of 0.2% (v/v).
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Soil material was also sterilized by γ-irradiation in order to obtain sterile samples for validating the passive IRT procedure.
In order to evaluate the effect of sterilization process on the viscoelastic parameters, the oscillation tests were repeated on sterile samples, which were obtained by autoclaving at standard conditions (121°C, 15 min).
In sterile samples, rewetting did not result in heat production.
Sterile samples were immersed in 0.9% (w/v) NaCl solution in airtight test tubes (Sterilin, Barloworld scientific, Staffordshire, UK).
An undisturbed sediment core taken from Round Lake, Hawke's Bay, New Zealand, using a specially designed sterile sampler, has been dated by tephra analysis and radiocarbon and analysed for pollen, charcoal and DNA.
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