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Soil material was also sterilized by γ-irradiation in order to obtain sterile samples for validating the passive IRT procedure.
In sterile samples, rewetting did not result in heat production.
In each plot, sediment samples were collected by randomly taking 5 soil cores using a sterile sampling device.
The samples were collected in 500 ml sterile sampling bottles.
Non-sterile samples were used as controls for all studies.
*From week two onwards, on day 1 of each week the contents of each fermenter were removed to a sterile plastic pot.
Place them in six-inch pots filled with sterile potting soil and fertilizer, and press them about 1/2 inch into the soil; keep it moist but not soggy.
Water samples were collected in sterile plastic sampling bags.
The seeds were sown into sterile soil pots, containing a mixture of vermiculite and organic manure (1 1, w/w).
Tobacco plantlets (Nicotiana tabacum var. White Burley) were grown in sterile magenta pots containing MS agar supplemented with 1.5% sucrose.
Expressed breast milk (EBM) was obtained using a sterile expressing kit into sterile plastic pots.
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