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Most wasted their lives in sterile sacrifice.
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To induce autoimmune hepatitis, mice were injected intravenously with Concanavalin A (20 mg/kg) or vehicle (sterile saline) and sacrificed 10 h later for blood and tissues harvesting.
Slices of full thickness cartilage were removed from the rabbits articulating joints, namely, the humeral and femoral heads, and the femoral condyles, using a sterile scalpel, immediately after sacrifice.
Sham control mice underwent the same procedure with administration of 100 µL of sterile PBS and were sacrificed the following day.
For the SDS-PAGE of animal samples, wild-type mice were infected intravenously with Listeria or injected with sterile saline solution and sacrificed after 72 hr.
At various time points, spleens and lungs from 4 mice were removed rapidly after sacrifice and a sterile autopsy was performed.
Blood samples were obtained from the right ventricle via a left anterior thoracotomy at the time of sacrifice, using a sterile heparinized syringe containing 50 μl of heparin (100 USP Units/ml).
Mice were injected intravenously with 5×105 CFU BCG in 200 µL sterile protein-free PBS and sacrificed at day 21 after infection.
D4 or D5 CPS vaccinated mice were i.p. injected with EdU labeling reagent (10 ml/kg body weight, Invitrogen, C-10418) dissolved in sterile PBS 2 hr prior to sacrifice.
Burned mice were subsequently housed individually in sterile cages and fed ad libitum until sacrifice.
All individuals were sacrificed by decapitation using a sterile razor.
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