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There was no differentiation between fertile or sterile elements within the androecial whorl, nor was there a combination that seemed to define petaloidy regardless of its whorl of origin.
In accordance with the expression pattern of B- and C-class genes described by Almeida et al. (2013), our present data do not show differential expression patterns between fertile and sterile elements within the androecial whorl, and neither set of results can explain the observed petaloidy or how, mechanistically, the staminodes and the fertile stamen show distinct morphologies.
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Epigenetic mechanisms involving chromatin modification, activation or silencing by sterile genetic elements and repositioning of var loci in sub-nuclear compartments also play important roles in the switching and exclusive expression pattern of var-genes [15] [21].
Portions of this medium were supplemented with 2 mL/L sterile trace-element solution and separately autoclaved aqueous stock solutions of (NH4 2SO4, (NH4 2CO3, or urea.
Sterile filtered trace elements were transferred to the reactor aseptically.
The LiDCO™ system consists of electro-medical equipment, sterile medical disposable elements and a sterile injectate.
The primary conclusions from the results are focused on the quick diffusion of hydrogen, unlike oxygen, within the coal particles and that the hydrogen forms stable compounds with the sulfur and other elements the sterile content.
Additionally, 2.67 mL L−1 of sterile vitamin and trace element solutions, respectively, as well as 0.1 mL L−1 of sterile antifoam were added after autoclavation (121 °C, 20 min).
The distance from the cornea to the microscope was kept stable using a single-use contact element in sterile packaging, (TomoCap, Heidelberg Engineering, Heidelberg, Germany).
For the subsequent fed-batch fermentation, 4 L of feed medium containing 100 g L−1 glucose, 3 g L−1 potassium hydrogen phosphate, 0.5 g L−1 magnesium sulfate heptahydrate, and 2.67 mL L−1 of sterile vitamin and trace element solutions, respectively, as well as 0.1 mL L−1 of sterile antifoam was made.
The carbon sources, glucose or glycerol, were prepared separately as 10x stock solutions (200 g L−1) and added after autoclaving. 1 mL L−1 sterile-filtered trace element and 1 mL L−1 vitamin solution, prepared as explained in [ 27, 28], were also added to the media after autoclaving.
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