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Apomorphies of monophyletic Bourreria are the valvate calyx lobes, an extensive disc, and an additional sterile chamber in each endocarpid (i.e., fruit part).
The girl does not have to live in a sterile chamber, as the "bubble boy" did, and she is kept in fairly good shape by weekly injections of a synthetic version of the enzyme.
The "heart in a box" is a wheeled cart with an oxygen supply, a sterile chamber, and tubing to clamp onto a donor heart and keep it fed with blood and nutrients.
Like all Minimal art, Heywood's paintings rely on the energy the viewer brings to them, and in the four stark-white paintings on view here he creates a sterile chamber suitable for navel gazing or catnapping, depending on where you're at.
Fresh human and mouse SF cells were cultured on sterile chamber slides (n = 6).
To examine the cellular localization of the ESCO2 protein, cells were grown on sterile chamber slides (Nunc, Roskilde, Denmark).
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Three weeks after surgery, mice were screened, and only those with sealed subcutaneous chambers containing sterile tissue chamber fluid (TCF) were selected for the experiments.
The sterile tissue chamber was then implanted subcutaneously under abdominal skin and maintained in the mice for 7 days to ensure the chamber was fully integrated with the subcutaneous environment.
For the immunostainings of the primary CAFs, ∼2500 fibroblasts were seeded on a gelatin coated, sterile, eight chamber glass slide (Lab-Tek II, Nalge Nunc Int, Roskilde, Denmark) and allowed to attach for 24 h in incubator.
The artery connected to the hypotube was placed inside the sterile glass chamber containing media (DMEM, 10% FBS, and 2% ABAM) and transferred to aluminum cage set at 37°C.
Upon adding the "pooled contaminated secretions" above the cuff, microbes could access the sterile "lung" chamber below the cuff either by gravitationally driven leakage between the cuff and trachea (simulating microaspiration) or by microbial proliferation along the ETT around the cuff.
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