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ZGZ and LD obtained the female sterile addition line.
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Post sterile additions for a batch medium were prepared as concentrated stocks, filter sterilized, and injected into the bioreactor through a septum on the head plate.
To each liter a post-sterile addition was made of: 1.0 mL 1000× vitamins, 1.0 mL 1000× trace metals, and 45.0 mL 20% glucose (final conc. 9 g L-1) [ 69], a formulation hereafter referred to as low-nitrogen, minimal medium.
Following aseptic addition of 6-8 sterile 3 mm diameter glass beads, specimens were vortexed for 30 seconds to homogenize the specimens.
Standardization of the inoculum to McFarland standards was by adjustment of culture turbidity by sterile saline addition.
The female sterile disomic addition line with one pair of the O. violaceus chromosomes (designated as S1) and donor B. napus cv.
The female sterile disomic addition line (S1) with all 38 chromosomes from B. napus and two copies of the chromosome from O. violaceus produced the progenies with similar phenotype, except the different female fertilities, after it pollinated donor B. napus (H3).
Whole yeast cells were kept in a sterile container, without addition of nutrients, at 4 °C and saturation humidity, during six days [6].
These observations indicated that in mice immunized with P. yoelii IrrSpz, a cross-reactive anti-CS immune response component contributes to cross-species sterile protection in addition to the non-CS cross-reactive one.
In addition sterile uterine inflammation may also explain the discrepancies.
In addition, sterile ns259 homozygous animals possessed AMsh glia of normal morphology, as assayed by expression of a glial F16F9.3 promoter:: dsRed transgene (n = 16).
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