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In destructive distillation the wood is heated by a stepwise raising of temperature in closed ceramic or stainless-steel chambers.
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The tissue was immersed for 72 hours in acetone containing 0.5% uranyl acetate as fixing agent at −90°C and the temperature was then raised stepwise 4°C per hour to −45°C.
The temperature was raised stepwise in 4 °C increments per hour from −90 to −45 °C, where it was kept for the subsequent steps.
Cells were originally grown in media containing BS-194 at 40 n M. When cells achieved 80% confluency, they were split and grown with raised stepwise concentration of BS-194 up to 200 n M in HCT-116 cells and up to 1.2 μ M in MCF7 cells.
Furthermore, the thermal stability of silver hydride species and the temperature dependence of the line shape of the peak due to acidic protons were studied with raising the temperature stepwise from 298 to 513 K.
After raising [Ca2+] in a stepwise manner to 1.0 mM, the K-H solution was replaced with tissue culture medium (M-199, Invitrogen, Karlsruhe, Germany) containing 1.25 mM [Ca2+] and supplemented with 20 IU/l human insulin, 0.2% (w/v) bovine serum albumin, 70 µM streptomycin, 100 IU/ml penicillin, and equilibrated with 100% O2.
However, the transition of the process to the stepwise mechanism upon increasing the driving force by raising the scan rate was established.
A simulation model of stepwise tumorigenesis illustrates how powerful clonal selection can be by, in effect, raising the mutation rate through increasing population size.
The infusion protocol required initially a slow drip rate which was raised at stepwise increments until reaching the target of 2 mg/min.
The stepwise approach, as proposed by our group, might be an effective first step towards raising the quality of care related to troubleshooting for intrathecal drug delivery with implanted systems.
"Raising sheep.
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