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For example, a potent macrophage-activating factor, Gc-MAF, is obtained from serum vitamin D binding protein (VDBP) by stepwise processing of the oligosaccharide attached to Thr 420 to the core α-GalNAc moiety.
In agreement with the stepwise processing of pre-miRNA precursors, 21 25 long oligonucleotides often originated from both arms of the hairpin, albeit at different frequencies: the mature miRNA outnumbers its cognate miRNA* sequence with a ratio up to 1713 3 (locus12, Figure 3A).
Although this is a useful model, it is clear that the stepwise processing is quite complex.
The temporal profiles for the ratios of Aβ40/43 and Aβ38/42 were simulated based on the stepwise processing model.
MiRNAs are generated by stepwise processing of RNA polymerase II (Pol II -dependent prII -dependentranscriprimaryi-miRNAs).
miRNAs are produced from a larger transcript through stepwise processing by ribonuclease III-like endonucleases in the nucleus and cytoplasm [ 17- 19].
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When βCTF supply is balanced roughly in the order with γ-secretase processing rate, the stepwise-processing model was found to have the two successive steady states, with each accompanying linear changes in [ES] or [S] concentrations.
The adjacency grouping is a stepwise process.
These results add another level of complexity to the molecular mechanics that are involved in the stepwise autocatalytic processing of the prodomain and imply a role for the auxiliary proteins and associated factors in these mechanisms [34].
The N-terminus of V3C18 undergoes stepwise proteolytic processing, involving release of a 50 kD biologically active precursor consisting of DUF-959, FZC18 and part of Tsp-1C18, that we called V3Nter.
Proteases such as saspase (17) and caspase-14 (18– 21) are implicated in the stepwise proteolytic processing of filaggrin.
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