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Genetic studies have also elucidated some of the mutations that occurred in the stepwise emergence of clonal complexes.
Zhang et al. used the used the same MWG probe set as Wick et al. [ 11], which was used to determine the stepwise emergence of O157 H7 strains from an O55:H7-like ancestor.
The recent observation that multiple small-effect mutations might contribute to the stepwise emergence of drug resistance or to reducing the cost of acquired resistance, appears to reinforce this notion; such polymorphisms could be critical to the ability of the organism to survive very specific selective pressures that do not correlate with relative growth rate in vitro.
While the stepwise emergence of E. coli O157 H7 from an O55:H7-like serotype has been advanced [ 11, 14], the relationship among E. coli O157 H7 lineages and clades incorporating data from standard and more advanced genotyping methods has not been evaluated.
It can potentially explain the stepwise emergence of a trans-splicing system comprised of several RNAs from a single cis-splicing element (though the large complement of proteins is more difficult – see Collins & Penny 2005 Mol Biol Evol 22 1053), whereas the exon theory of genes was never a good explanation for the origin of introns.
This added 83 strains to the 19 strain in silico dataset and included data for three E. coli O55:H7 strains and two sorbitol-fermenting E. coli O157 H-strains (Table 2), allO157 H-strainsame Tableesulting supernetwork within the context of the proposed stepwise emergence of E. coli O157:H7 [ 11].
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Such multi-component signaling platform is the hallmark of multi-cellular animals, and is suggested to result from stepwise evolution during the emergence of metazoan species [ 12, 32].
The emergence of EcO157 has been described by a stepwise model, in which EcO157 evolved in a series of steps from O55:H7 by acquiring a stx2 gene, conversion to serotype O157, acquiring a stx1 gene and changes associated with conversion to SOR -and GUD-[ 27- 29].
We show that TEC progenitors can undergo a stepwise acquisition of first cTEC and then mTEC hallmarks, resulting in the emergence of a progenitor population simultaneously expressing the cTEC marker CD205 and the mTEC regulator Receptor Activator of NF-κB (RANK).
The emergence of 3 LIII subgroups is likely to be concomitant with stepwise genome reduction as observed in some non-pathogenic Listeria species, including L. welshimeri [ 48] and L. seeligeri [ 49].
Combined, these patterns suggest an evolutionary stepwise acquisition of the tripartite mammalian C-peptide molecule, with insulin-interaction being ancestral, various turn stabilizations apparently of intermediate emergence, and possible receptor-interaction the most recent addition.
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