Sentence examples for stepwise elution from inspiring English sources

They were then transferred to the column and eluted as above except the stepwise elution gradient was comprised of 500 ml of 0 1, 1 1 and 1 0 (v/v) CH2Cl2: hexane and finally MeOH, and 2.5 ml fractions were collected.

The purification procedure, which involves anion exchange and affinity chromatographies, was performed as previously described by Girbal et al. [ 7], except that during the stepwise elution from Q-Sepharose, the algal hydrogenase eluted at 0.25 M NaCl.

Instead, stepwise elution is usually used.

In this paper, a design calculation method for stepwise elution proposed previously was modified and extended.

The pH stepwise elution and sample loading were optimized to improve the IgG purification from serum albumin containing feedstock.

Stepwise elution experiments were also designed and performed successfully on the basis of the K–I curve.

The chromatographic programme was modified to three stepwise elutions in order to improve the separation of cellular fractions (Fig. 1).

The nonadsorbed protein fraction was eluted from the column with starting buffer (100 mL), and the adsorbed enzyme was collected through 5-stepwise elution chromatography (sodium chloride concentration: 0.1 M, 0.15 M, 0.2 M, 0.25 M and 0.3 M in the same buffer).

The sample was loaded using 50 mM Tris HCl buffer (pH 7.0) at 5 mL/min flow rate and was eluted stepwise: 1st elution at 35%, and 2nd elution at 100% of 0.2 M sodium chloride in 0.05 M Tris HCl buffer (pH 7) (Jäger et al. 2010).

Peptides were fractionated and stepwise eluted using each time 500 µl of elution buffer (5 mM KH2PO4, 25% acetonitrile, pH 3, with the addition of 50, 100, 150, 200 and 350 mM KCl).

The flow-through containing unbound protein was discarded and a stepwise NaCl elution was used to elute the correctly folded dimer at 200 mM NaCl (H1) and the majority of soluble aggregates at 600 mM NaCl (H2).