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Nine analogs of scorpion toxin peptide κ-hefutoxin 1 were synthesized by stepwise deletion of its amino acid residues.
Similarly, the deletion of CDs in E. coli was shown to be effective for increasing the synthesis of cysteine, where the stepwise deletion of known CDs decreased the intracellular CD activity, resulting in increased cysteine synthesis proportional to the number of deleted genes (Awano et al. 2005).
For example, Scholl et al., show that stepwise deletion of MEK1 or MEK2 alleles reduce Ras-induced epidermal hyperplasia to a similar extent [42].
The contribution of each explanatory term was tested sequentially, with non-significant terms removed from the model to produce the minimal model following standard stepwise deletion [43].
The most parsimonious model was selected by stepwise deletion of non significant terms from the maximum model by using Akaike's Information Criterion.
For each measure of disease incidence and flock susceptibility, model construction proceeded by stepwise deletion of non-significant terms from an initial model including flock-level susceptibility, flock size and flock type.
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Stepwise deletions of the terminal helices of PNGase were performed to further analyze the functional roles of the terminal residues.
The region around GLI3 is prohibitively large for using the painstaking strategy of stepwise deletions in reporter gene assays.
To investigate the role of this enzyme in this pathway, we made stepwise deletions of the N-terminal helices of peptide N-glycanase.
We built multiple linear regression models using stepwise backwards deletion and including main effects only (no interaction terms).
Variance explained by each explanatory factor and levels of significance were calculated by using a stepwise backward deletion test (28 ).
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