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We made stepwise adjustments of potential confounders in the data analysis.
The stepwise adjustments for the most significant SNP effects led to a further reduction of λ to 1.4.
provided that stepwise adjustments are made until beta-globin PCR positivity is obtained and primers are chosen which amplify a maximum of about 200 bp.
No additional SNP was identified after the stepwise adjustments for the effect of these significant SNPs (see Additional file 2: Table S1).
Stepwise adjustments of traditional slow freezing protocols as well as optimization of minimum volume vitrification methods (Arav, 1992; Kuwayama et al., 2005; Kuwayama et al., 2007) have resulted in breakthroughs in this field.
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Whereas crude and unadjusted analyses suggested an association of colloids with adverse outcome, a stepwise adjustment for baseline and progress covariables indicates that the use of colloids per se did not affect the risk of mortality.
Three additional stepwise adjustment models were developed for sensitivity analyses.
All associations remained non-significant with stepwise adjustment for age and waist-to-height ratio.
$Multivariate correction for sex, age and underlying disease (forward stepwise adjustment) using logistic regression analysis.
Covariates including age, gender, sepsis and SOFA were used for stepwise adjustment.
Stepwise adjustment for age and sex, waist, serum insulin, triglycerides and diastolic blood pressure was used.
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