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Both steps were highly important because they reduced the amount of nuclear DNA contamination.
Both purification steps were highly efficient (Fig. 1B) and led to the detection of several proteins that were isolated with the TbAUK1-PTP fusion protein (Figs. 1C and 1D).
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The key steps are highly efficient binary ligation under conditions where the vector is relatively dilute, with only a modest molar excess of insert, followed by efficient electrotransformation into Escherichia coli.
The cell alignment and electroporation steps are highly related to the intensity and distribution of the electric field, which depend on the applied voltage as well as the microelectrode structure.
While the first two steps are highly dependent on the input data and selection of appropriate modeling algorithms, the third step is an integration of results of the first two steps.
Notably, all these steps are highly connected to each other and linked with other gene regulatory layers to ensure proper expression of every gene in a cell [3].
These steps are highly amenable for genetic manipulations to increase the oil content.
Third, daily steps are highly interpretable 20 by both scientific and lay communities.
Although some diagnostic steps are highly standardized (e.g., database searches, clinical utility gene cards, and standard clinical scores), others are not suitable for standardization.
All these steps are highly regulated by a number of evolutionary conserved autophagy related genes (ATG genes) and ubiquitin-like conjugation systems.
Each of these three steps is highly configurable by the choice of elementary processing components that best fit the task at hand.
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CEO of Professional Science Editing for Scientists @ prosciediting.com