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The in situ pre-treatment and hybridization steps were essentially carried out as described [ 22].
12 As all of the required reagents for hydrazide and thioester formation are salts, protein-handling steps were essentially performed in a one-pot fashion with the aid of a centrifugal filter (with a cut-off molecular weight of 3 kDa) to exchange buffers between reactions.
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These two steps are essentially separate, enabling great control over the final morphology of Ag nanostructures [13, 67].
The posthybridization washing steps are essentially those described by UltraGAPS Coated Slides instruction Manual.
The purification steps are essentially the same as non-lipidated Gβγ.
The steps are essentially the same as they were with the first half, except in reverse.
What you did in the previous steps was essentially replace the information the file available to you (CTF, or Folder 32) with information from a file unavailable to you (Team Slayer, or Folder 34).
The next step is essentially a relabeling process, where we convert that impulse train simply to a sequence.
Thus the minimization of the objective function f on the 2D subspace S ( x 0, g ~ ) and that along the projected gradient step are essentially the same.
Parallel-distributed hybrid BFS is similar to the sequential algorithm in Fig. 4, heuristically switching between top-down and bottom-up per each iteration step, being essentially a hybrid of algorithms in Figs. 5 and 6.
But beyond that, the next step is essentially we do this process probably once every two months where we just all get together and go through essentially all of the ideas that we have or that people have suggested and figure out what we think is the next step for the next couple of months.
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