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All steps were achieved at 4°C.
Additional purification and separation steps were achieved by adsorption and gel permeation chromatography.
These steps were achieved using to the EBImage R package (Pau et al., 2010).
These trimming steps were achieved using internal software based on the FastX package [ 29].
These trimming and removal steps were achieved using in-house-designed software based on the FastX package [ 21].
The remaining two chemistry steps were achieved using a simple two-step flow procedure with only minor modifications to the first-generation conditions (Scheme 3).
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Stability for arbitrary time steps is achieved by allowing waves to propagate through more than one mesh cell in a time step.
Through first principle calculations, every species and energy barrier for elementary steps are achieved, and formate ion is determined as the important intermediate.
In organisms that metabolize starch or glycogen, the degradative and phosphorylating steps are achieved either concomitantly by an ATP-independent pathway or separately by an ATP-dependent pathway in which phosphorylation is catalyzed by hexokinase (for reviews, see [108], [109].
All subsequent re-engineering steps are achieved by simple co-transfection of ESCs with a custom-designed RMCE vector and also the Flp expression vector.
In contrast, both of these steps are achieved simultaneously for a BES which only requires traversing the lesion with a microcatheter once.
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