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The concentrations of antibodies used and washing steps were according to the manufacturer's instructions, all antibody incubations were with 50 µl/well.
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The specific operation steps was according to the previous study24.
The following steps are according to the standard SeqCap EZ protocol (Roche NimbleGen, Inc).
In the two-step scheme, the optimum width of transmitted pulses in first step is according to (14).
The primary step is, according to guidelines, switching from the causative agent to an SGA followed by, if necessary, additional pharmacological treatment.
The scoring for the first step is according to the standard PETfold approach, where we use thresholds for reliable base pairs that have been identified according to training on more than 30 verified interactions in bacteria, which is described later.
District officials have not yet decided what the next step will be, according to Marge Rader, Glendale Unified's administrative secretary said.
The processing steps were performed according to the following protocol.
The titration, emulsion PCR, and sequencing steps were performed according to the manufacturer's instructions.
All the cloning steps were done according to Sambrook et al. (1989).
All these steps were performed according to the manufacturer's protocol.
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CEO of Professional Science Editing for Scientists @ prosciediting.com