Sentence examples for steps samples from inspiring English sources

After dehydration, using an ethanol series (70 % - 70%% - 90%% - 96%% - 100%% - 100%%; two hours steps), samples were embedded in glycolmethacrylate (Technovit 7100; Heraeus Kulzer, Wehrheim, Germany) with the transversal side facing upwards.

After several washing steps, samples were viewed under high power microscopy (Leica).

Following serial ethanol dehydration steps, samples were mounted onto glass slides with ProLong Antifade reagent (Molecular Probes).

After two selective hydrolysis steps, samples were separated by reversed phase HPLC (Symmetry C18-column 4.6×150; 5 µm; 100Å; Waters) with an increasing gradient of buffered methanol/acetonitrile coupled to fluorimetric analysis (Van Veldhoven P.P., to be published).

Following subsequent washing steps, samples are incubated further with tetramethylbenzidine (TMB).

After washing steps, samples were mounted using fluorescence mounting medium (DAKO).

Conventional A/D converters consist of two steps: sampling operation followed by digital quantization.

It includes the following steps: Sample treatment → Acetone precipitation → Resuspend sample → Isoelectric focusing → Soak gel with SDS → Transfer and blot.

Geomorphometric analysis commonly entails five steps: Sampling a surface, generating and correcting a surface model, calculating land-surface parameters or objects, and applying the results.

For the final analysis, the program was run using 50 chains for a total of 5×107 steps (sampling every 100) following a burn-in period of 500,000 steps.

By incorporating the tags in two steps sample-handling complexity is lowered by nearly 100 times compared to conventional indexing protocols.