Sentence examples for steps reverse from inspiring English sources

The C-ORF technique consists of three steps: reverse transcription, dSLAP annealing plus the second-strand synthesis, and PCR amplification.

Although mutations can be introduced at any of these replication steps, reverse transcriptase (RT) errors play a major role in retroviral mutation.

The human panel early access kit (P/N: 4365381, Applied Biosystems) used in this study contained 160 individual assays and comprised two steps: Reverse Transcription (RT) and real time PCR.

on the Light-Cycler® 480 real-time PCR system (Roche Diagnostics, Basel, Switzerland) according to the following steps: reverse transcription at 42°C for 10 min, denaturation at 95°C for 10 s and 40 cycles at 95°C for 5 s, 55°C for 20 s, and 72°C for 10 s, followed by a cooling step at 40°C for 10 s.

Pre-amplification was carried out using the following steps: reverse transcription at 50 °C for 15 min, denaturation at 95 °C for 2 min, 24 cycles of amplification (95 °C 15 s, 60 °C 4 min) on a C1000 PCR instrument (Biorad).

Students could mutate the experiments by: deleting certain experimental steps in the standard protocol (namely deletion mutation), adding extra steps (insertion mutation), reversing the order of experimental steps (reverse mutation), or replacing certain steps (substitute mutation).

Repeat those steps, reversing the direction of the diagonal each time, until you have what looks like a zig-zag all the way down the loom.

In the next step, reverse transcription of such an edited RNA results in a DNA sequence that is inserted into the DNA strand instead of the damaged fragment.

For chowder, add another step: reverse the knife and run the dull side down the ear to press out the rest of the corn and its milk.

They said that Mr. Pitt, who last month personally informed Mr. Biggs that he supported him for the job after telling the other commissioners that he intended to take such a step, reversed himself last Tuesday.

"We needed to take definite steps to reverse the trend.