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Mouse primary hepatocytes (male, 6 12 weeks old), were isolated by a standard two-steps retrograde perfusion procedure [ 23] and purified using percoll gradient [ 24].
Mouse primary hepatocytes (male, 6 12 weeks old), were isolated by liver perfusion of Rb-floxed mice (homozygous for exon 19 of Rb flanked by LoxP sequences)[ 25] p53-/ [ 26] and p21Cip1-null mice [ 18] using a standard two-step retrograde procedure [ 27].
After the transport of the toxins into the TGN, the next step in retrograde transport is the transport through the Golgi and to the ER.
This step of retrograde transport can be readily studied in cells treated with BFA, which induces the formation of long Golgi membrane-tubules along microtubule tracks that rapidly fuse with ER membranes (Sciaky et al., 1997).
Putin's retrograde steps mask deeper troubles.
While not easy, the West has taken steps to counter retrograde Russia.
The CCC found that other major emitters were also making considerable strides forward in cutting emissions - despite slow progress on global talks and some nations (namely Australia, Canada and Japan) making retrograde steps.
The EU Commission and MEPs say they regret "retrograde steps" in the Balkan nation which are delaying its progress towards EU membership.
Similar data were collected for subsequent retrograde steps.
Thirty-one (66%) patients on ASB progressed on CPAP without any retrograde steps.
Twenty-nine (62%) patients on PCV progressed on ASB without any retrograde steps.
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CEO of Professional Science Editing for Scientists @ prosciediting.com