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Early steps of virus life cycle, including virus entry, nuclear localization of NP and viral RNA-dependent RNA replication, were not affected by KR-23502.
Understanding the fundamental steps of virus life cycles including virus host interactions is essential for the design of effective antiviral strategies.
Cell surface proteins called viral receptors mediate the initial steps of virus attachment and uptake.
Furthermore, we have taken advantage of the flexibility of pseudotyped particles to investigate the molecular mechanisms underlying initial steps of virus entry following cell attachment.
In addition to the possible use of these compounds as antiviral agents, they could also be useful to understand various steps of virus replication and/or virus-host interactions.
Our findings pave the way towards the design of new drugs exhibiting Arb-like interfacial membrane binding properties to inhibit early steps of virus entry, i.e., attractive targets to combat viral infection.
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Viral attachment and entry, representing the first steps of virus-host cell interactions, are major targets of adaptive host cell defenses.
Investigators have identified the amino acid sequences of the PB1, PB2, and PA proteins that function in specific steps of virus-specific RNA synthesis or in mediating interactions between the three proteins (2).
Among all the antiretroviral molecules, antibodies occupy a special position as they can inhibit HIV-1 replication by interfering with multiple steps of virus-cell interaction.
This in turn induces peroxisome-mediated synthesis of lipids called plasmalogens, which enhances virus infectivity at the step of virus assembly.
We have also excluded the possibility that both inhibitors act at the replication step of virus life cycle.
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