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The steps of the validation of this process prior to its implementation at a production scale are covered in the paper.
The strength of this study is the two steps of the validation procedure.
All steps of the validation procedure proved that the developed analytical procedure was suitable for its intended purpose.
All steps of the validation procedure proved that developed analytical procedure was suitable for its intended purpose.
We next set out to determine the uniformity of amplification using fully validated PrimerBank primer pairs ie. primer pairs that had been successful in all steps of the validation procedure.
Given the low Cronbach's alpha and ceiling effects, it is recommended that the Brazilian version of the PedsQL™ Oral Health Scale be tested in dental clinic samples, including children with more severe dental problems as the next steps of the validation process in Brazilian children and adolescents.
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As a last step of the validation, the issues identified are corrected through discussed among the task analysts and SMEs.
Specific inconsistencies that were found during this step of the validation pertained to differences in terminology used in medical literature, especially across specialties.
In order to validate the proposed quality metric, we proceed as follows: Quality criteria behavior with alterations: the first step of the validation process consists of showing the robustness of the used five quality criteria in detecting the introduced alterations: blurring, Gaussian noise, and resize alterations.
The first step of the validation was to test if the 109 samples selected for stereological point counting were representative for the whole set of 198 samples.
As shown in Figure 2, the first step of the validation process was optimization of primers and primer mixes for specific gene targets on the chip.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com