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The single steps of the sample production are illustrated in the schematic sketch in Fig. 1.
We note that this approach would preclude real-time detection and require further processing steps of the sample, which detracts from the strength of label-free detection.
To avoid possible sources of variation in the data, individual laboratories developed standardized protocols involving all the various steps of the sample preparation procedure, starting from tumor sample collection, through sample processing, total RNA isolation, cDNA synthesis, cRNA synthesis and labeling, target fragmentation, microarray hybridization, to washing and staining protocols.
This partitioning of variance allows the removal of systematic error (which in the case of metabolomics data may easily arise from systematic or random error at various steps of the sample preparation or analysis) that may interfere with the regression of the response variable, hence the method's suitability to metabolomics data analysis.
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General steps of the sampling and analysis process for whole grain, as well as sampling terms (Codex Alimentarius 2009) are shown in Figure 1.
The recoveries of the combustion and transfer steps of the samples and standard reference materials ranged between 89 100%, and 92 106%, respectively.
This highlights the importance of controlling every step of the sample preparation process for the well counter.
This paper maps the silk fibroin molecular conformations of each step of the sample preparation and the electrospinning process.
A new method which avoids the drying step of the sample has been used to give images of individual xanthan molecules.
As we add the internal standard in the first step of the sample preparation process, not only changes during chromatography but also during extraction (e.g. due to differences in ethanol content for the herbal medicines) are corrected.
To prepare low hematocrit samples, erythrocytes were diluted to the desired concentration during the final washing step of the sample preparation.
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