Sentence examples for steps of the purification from inspiring English sources

The most favorable parameters, which provide a complete carbon disulfide removal from air, are determined for all steps of the purification process.

The homogeneity of the sample was followed through all steps of the purification by SDS-PAGE.

The major steps of the purification protocol are depicted in Fig. 2. Briefly, the first step was performed by adding ∼5 mg of M-270 Epoxy Dynabeads (Invitrogen), with immobilized anti-Flag antibody (M2, Sigma), to 5 7 ml of a crude cell extract.

All steps of the purification were performed at 4 °C.

Fractions collected during different steps of the purification were analyzed by SDS-PAGE.

However, their presence in the initial steps of the purification could truly influence the GC composition of the purified RNAs.

The general step of the purification using NGC Quest™ 10 system was as follows.

Enzyme activity (ABTS assay) and total protein concentration by Lowry's method was estimated at every step of the purification.

At each step of the purification, the concentration of 2G12 was monitored by surface-plasmon resonance (SPR; Table 1).

After removing fat and casein from the milk, a cation exchange column was used during the first step of the purification method to remove most of the whey protein.

In order to achieve higher purity (>95%), the fractions containing rHLZ were further purified in the second step of the purification process by gel-filtration chromatography in 0.15 M sodium chloride with 20 mM sodium phosphate buffer, pH 7.2.