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The quantification of bacterial contamination necessitates the assessment of the efficiency of the used methodology as a whole, including the preceding steps of sampling and sample processing.
In this work the authors examined the three basic steps of sampling, subsampling and chemical analysis that are employed to measure mycotoxins in food.
In addition, for increased sensitivity, methods with the highest possible efficiency should be utilised and the dilution factor in all steps of sampling and sample processing kept to a minimum.
Moreover, for a reliable quantification of bacterial contamination, it is important to assess the efficiency of the used methods as a whole, including both the molecular detection method as well as the preceding steps of sampling and sample processing [ 3– 5].
Although the geographic distribution and socioeconomic status were taken into account in the sampling process, the initial two steps of sampling were not random.
Although considerations of the geographic distribution and socioeconomic status were taken in the sampling processes, we should acknowledge that the initial two steps of sampling were not random, and our choice of those counties was mainly based on the former collaboration between local health bureau and China Institute of Health.
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This could also avoid the previous mandatory step of sampling.
The packaging and delivering steps were performed by a research assistant who was blinded from every step of sampling and analyzing.
Finally, to compare model results with animal blood data, we must consider the final step of sampling small aliquots of the differentiated blood.
As an important and necessary step of sampling biological specimens, the separation of malignant cells from a mixed population of cells usually requires sophisticated instruments and/or expensive reagents.
A laboratory fortified blank takes into account all steps of sample preparation and analysis.
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