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Plugging (15) into (11) and following similar steps of manipulation in [13], we have the update for the parameters begin{array}rcl@ lambda_{k}^{t+1} &=& frac {1}{M} Sigma_{m=1}^{M}{pi_{km}^{t}}, end{array} (17a).
CFGE combined with Cre/lox recombination is put forward as powerful approach for targeted genome editing, involving serial steps of manipulation to expedite the genetic accessibility of S. meliloti as chassis.
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With several steps of manipulations as detailed in the Appendix, the problem is finally casted to a standard form of GP.
However, introducing mutations at multiple sites requires multiple steps of DNA manipulation, which is time-consuming and labor-intensive.
A disadvantage to this approach is that it requires several steps of genetic manipulation and has only been successfully used in T. brucei.
After the reaction, light- and heavy-labeled cell lysates were mixed prior to any further steps of sample manipulation as described above.
In this study, we specifically chose not to deplete the highly abundant proteins from the Pap test samples prior to MS, since our goal was to see whether it would be feasible to perform a limited number of steps of sample manipulation and still identify hundreds of proteins.
We also showed the applicability of the proposed method to a step-by-step manipulation by sequentially inputting random targets.
A paired-end library was constructed according to the manufacturer's protocol, with modifications and optimizations to enable robotic automation of crucial steps and manipulation of limited, high-% AT starting material.
However, the multiple steps of post-PCR manipulation in these procedures require time and pose the risk of DNA contamination.
Whereas full-length cDNA microarrays were the first choice for the generation of microarray platforms, oligonucleotide-based chips are gradually gaining importance due to the reduction of manipulation steps and their ability to differentiate similar members of gene families [ 23].
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