Sentence examples for steps of heating from inspiring English sources

An aliquot of 3  μg total RNA in 100  μl diethyl pyrocarbonate (DEPC) water was processed through serial steps of heating at 65 °C for 5 min, cooling on ice for 5 min, incubating for 1 h in a solution at 4 °C, and purification by Microcon 30 before RT-PCR.

All antibodies required a pretreatment antigen-enhancing step of heating the slides in 95°C 0.1 mol/L citrate buffer at pH 6.0 for 10 minutes before incubation with the primary antibody.

(3) The QIAamp Stool Mini Kit procedure included the manufacturer's optional pre-treatment step of heating samples in a water bath at 95°C for 10 min, with a prior modification of pre-filling tubes with glass beads and ASL buffer, and disrupting the cells using a Precellys®24 ribolyser at 5500 cycles per min for 30 sec.

The optional step of heating the lysis solution to 65 °C was used to maximize RNA yield.

It is thus clear that the extraction phase should not be conducted using a routine process and must comprise a supplementary step of heating to 90°C for 1 h to improve bisulfite conversion.

The standard amplification program included 40 cycles of two steps each of heating to 95°C and 60°C.

The antigen retrieval step consisted of heating the slides in EDTA buffer (pH 7.8) at 95 °C for 45 min.

By comparing experimental results and simulations for upward and downward steps of heat load, the response mechanisms within the loop can be analyzed.

In contrast, in the early steps of heat stress, the induction of caspase-dependent apoptosis was related to a relative up-regulation of Bcl-2 consecutively followed by a delayed decrease in apoptosis activity.

From thermal analysis, the addition of kenaf fibre reduced the degree of crystallinity of the r-PP/PE but an improvement can be observed after the second step of heating and cooling.

The amplification parameters were 40 cycles of two steps, each step comprised of heating at 95°C and extension at 60°C.