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A. aegerita aromatic peroxygenase (Aae APO) was purified from cultures of A. aegerita strain TM-A1 (deposited at the Leibnitz institute DSMZ - German Collection of Microorganisms and Cell Cultures; collection number DSM 22459) by several steps of fast protein liquid chromatography (FPLC) as described previously (Ullrich et al. 2004).
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The two main steps of FaST-LMM Select are ranking SNPs by linear regression P-values to form the GRM with the top-ranked SNPs and then calculating association statistics in a mixed-model framework, using this GRM.
When the spraying operation was stopped, the visibility re-increased in two main steps: a first step of fast increase and a second step of slow increase needing a few tens of seconds to get again a fully stratified smoke layer.
The first step assumes slow continuous nucleation followed by a second step of fast autocatalytic surface growth.
A first step of fast insertion/dissociation of the dye into and from the plasma membrane (half time of 1 to 2 s) was followed by a slower step where transfer from the plasma membrane to the lipid droplets occurred (half time of 30 s to 2 min).
The steps of the fast selection algorithm are illustrated in Algorithm 6.
(E) State of evolution fast LF at Nit=80 and PDE approximation at T=706.8. Figure 4 shows the dice coefficient between the first 80 steps of the fast LS evolution and the corresponding state of the numerical approximation.
The quadrant which lies in the negative I and Q sectors of the leakage signal is only slightly affected, since here the probing signal and the leakage signal partially cancel each other and result in a low amplitude signal which is measured in the second and third steps of the fast algorithm.
He blames "the goose-step march of fast-food restaurants southwards".
DNA from ancient microbes could also help today's medical researchers keep one step ahead of fast-evolving diseases like cholera and influenza.
Kiesewetter had a pair of deft crosses that nearly led to goals — including one deep in the box that was just a half-step ahead of fast-closing Morris — before using his speed to draw the foul that set up the winning goal.
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