Sentence examples for steps of enzymes from inspiring English sources

There were three steps processing the raw BSG and PM: (1) thermochemical pretreatment, which broke down the structure of raw materials to increase the solubilization yield; (2) enzymatic hydrolysis, which was performed by various steps of enzymes under different pHs and temperatures; (3) filtration, which separated the liquid and solid fraction in the last step.

The steps of enzyme concentration and ester synthesis were performed by applying central composite rotational designs (CCDR) in order to evaluate the process variables.

We discuss path sampling methods to measure rates of elementary steps of enzyme action, coarse-grained modeling for understanding macromolecular, cellulosomal complexes, methods to screen for enzyme improvements, and studies of cellulose at the molecular level.

The lack of observable deuterium isotope effects offers a cautionary note for the application of caged substrates to isolate and study chemical steps of enzyme reactions, particularly when NADH is involved in the reaction pathway.

Two redox couples (−0.481 and −0.605 V vs. SCE in Tris buffer solution at pH 7.5, scan rate 20 mV s−1) were obtained on the cyclic voltammogram which correspond to the separated two peaks in the one-electron reduction-reoxidation steps of enzyme bounded flavin mononucleotide (FMN).

The 2-amino-3-hydroxy-cyclopenta-2-enone production through 2 steps of enzyme reaction was reported previously (Zhang et al. 2010).

To construct a plasmid for OsWRKY80 RNAi, part of the WRKY80 cDNA (302 bp, nucleotides 775 to 1076) was amplified by PCR and used to construct self-complementary hairpin vector pCo-Ubi: dsWRKY80 after several steps of enzyme digestions and ligations.

The synthesized aCD138-ScFv and aCD3-ScFv were successfully inserted into the pFUSE vector after two steps of enzyme digestion and ligation using NcoI, BamHI, and BglII cloning sites (Fig. S1).

Combining the steps of enzyme production and sugar fermentation in a one-step process called consolidated bioprocessing (CBP) has the potential to address this limitation but requires the development of an organism that both degrades cellulose efficiently and produces fuel at high yield and titer [ 4].

After several steps of enzyme-coupled reactions with 10-acetyl-3, 7-dihydrophenoxazine (Amplex Red reagent), alkaline phosphatase, choline oxidase and horseradish peroxidase, PC-PLC in whole-cell lysates and serum converted PC (egg yolk lecithin) substrate to highly fluorescent product resorufin.

The step of enzyme hydrolysis has been optimized using response surface methodology for concentrations of the enzymes and the concentration of biomass.