However, considering structural design feasibility and remote handling compatibility, only the first two configurations were selected for the first step of engineering design.
Computer-assisted pathfinding has the potential to save biochemists time in the initial discovery steps of metabolic engineering.
Figure 1 illustrates the workflow of SimBoost and SimBoostQuant, consisting of the three steps of feature engineering, gradient boosting trees and prediction interval.
Crude oil and gas properties are used in various steps of petroleum engineering in order to evaluate oil and gas reserves, recovery efficiency, production optimization, etc.
This was accomplished by three steps of genetic engineering.
This is possible because of the conservation of the signal recognition- and ER-insertion mechanisms throughout eukaryotes [ 17, 28, 29] and circumvents laborious steps of signal engineering.
Based on strain Z1978, we generated strain Gen III HP Z5567 (Hong et al. 2011a) by six more steps of metabolic engineering.
With multiple steps of genetic engineering and adaptive evolution, we obtained the strain BSW3AG, which grows on L-arabinose with a μmax of 0.075 h−1.
The different steps of the engineering strategy are summarized in Figure 2. The initial phase of this work involved the use of error-prone PCR (epPCR) to generate random biodiversity.
Plenty of techniques are actually available at this scope and it is not always easy to understand how to select and use them in the different steps of re-engineering.
