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The CAP3 assembly program [ 66] was used to perform subsequent steps of clustering, sequence assembly, alignment analysis and consensus partitioning to derive contigs and singletons.
The steps of clustering are designed based on this approach.
The TE annotation pipeline used here contains a number of supplementary steps of clustering of the REPET outputs and allowed us to identify more complete elements.
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In this paper, we present a MapReduce-based Parallel Kaufman (MPK) implementation for accelerating the initialization step of clustering.
Our method adds to the calculation of similarity between couples, a step of clustering and partitioning.
This is followed by second step of clustering nonneighboring, similar sequence segments.
Clearly, the preliminary step of clustering genes into target modules was necessary here to infer the complete Hap4 regulated module.
As a final step of clustering analysis, the application of cluster validation techniques is vital to assist users in understanding some fundamental questions such as: Are these clusters biologically meaningful?
Beyond a first step of clustering, sequence similarity networks allow biologists to perform fine-grained analyses of similarities between sequences, because they exploit the information provided by the topology of weighted connections between sequences within and between clusters.
The algorithm of frequent probability subgraph discovered by two-step hierarchical clustering takes L c/2 as each step for the comparison (L c is the number of cluster), so that the new categories are the most likely to become the two candidate classes for pairwise comparison at next step of clustering.
See Fig. S1 (points 4 6) for details of the last steps of "cluster then converge" analyses: The sets of results from approaches (1) and (2) were compared to those expected by chance using 100,000 Monte Carlo II simulation trials (below) ([36] and below, and Johnson et al, in preparation).
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