Several studies have demonstrated that some C-type lectins may function as adhesion, signaling, or antigen-uptake receptors [ 32– 35], and these results are consistent with the fact that some CLRs are present on MØs and DCs, which play a role in the initial step of capturing the antigens containing carbohydrates [ 36].
The non-stationary sweeping procedure has the merits of adapting large load steps to capture static characteristics of stable equilibrium paths both before and after mode jumping and reduce automatically the step size to ensure a dynamic transition between the two stable branches.
Automatic shot classification can also serve as an important step in approaching the elusive problem of capturing semantics or meaning in the video sequence (see, e.g., [14]).
(An exhibition of them at the American Museum of Natural History was the occasion for Ms. Alexander's book.) The filmmakers, who retraced some of Shackleton's steps to capture the otherwordly landscapes he explored, also use the testimony of descendants of the crew and a few discreet and well-placed re-enactments.
Limousine companies are also taking steps to capture the new market of business travelers who drive.
In our experiments, we fix the distance between the CCD lens and the brake pad first, and then adjust the focusing distance with a constant step to capture two sets of images from low definition to high definition and from high to low definition again.
With this specific affinity medium, the enzyme recovery process consisted of only one chromatography step to capture 3α-HSD.
Owing to this fragility, the multiple processing steps involved in the CEK system, including additional washes and labelling steps, could cause degradation and loss of captured CTCs.
After 40 min of incubation at 60°C, the 100 μL hybridisation reactions were transferred to a KingFisher 96 magnetic bead particle processor (Thermo Electron) and the following steps of affinity capture, washing and elution were automated in 96-well plates.
Although the steps of substrate capture and product release are essential in the enzymatic process, complete atomic-level descriptions of these steps are difficult to obtain because of the transient nature of the intermediate conformations, which makes them largely inaccessible to standard structure determination methods.
