Sentence examples for steps of assay from inspiring English sources

Genomic DNA was prepared for use in the various steps of assay design and optimization using the QIAamp DNA Mini Kit (QIAGEN, Valencia, California) using Protocol C then quantified for use in standard curve experiments using a NanoDrop ND-1000 or 8000 spectrophotometer (Nanodrop Technologies, Wilmington, Delaware).

These samples were not considered during the third step of assay development (consistency check).

Optimisation of a MOL-PCR assay can be attempted at different parameters of the preparatory work and in each of the 3 steps of the assay.

Compared with the currently-used methods, this new method avoids complex treatments of lipid substrats/products, thus fewer steps of the assay procedure are required.

Although we found that slide chemistry did not directly affect the signal to noise ratio, few slide chemistries could survive the required extension and stringent wash steps of the assay.

All steps of the assay are performed at room temperature.

All the steps of the assay were done according to the manufacturer's instructions.

All steps of the assay were performed at room temperature (25°C).

All steps of the assay protocol were performed in RNAse-free conditions.

The subsequent steps of the assay are equivalent to the total anti-CII IgG ELISA, described in the previous paragraph.

We have created a modified version of the cartoon describing all steps of the assay, improved the labelling and added more detailed description.