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The lab work consisted of three major steps: Isolation; characterization; culturing and labeling of MSCs.
Production of clinical grade Tregs has three critical steps: isolation, expansion, and quality control.
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Two steps (isolation-enrichment and detection) are combined in most cases to identify CTC.
RNA from mouse organs was prepared as previously described using a single step isolation protocol [51].
In the second step, isolation of complete 5' LTRs was performed.
Total RNA was extracted from SW480 cells and purified microvesicles by a single-step isolation method using TRIzol reagent (Invitrogen Corporation).
The described genomic-proteomic approach facilitates the fast one-step isolation and identification of an unknown promoter-binding protein.
Our study also confirms that platelet concentrates may be successfully obtained through a one-step isolation procedure.
The surgical procedure for the isolation of primary hepatocytes is based on a modified two-step isolation method described by Seglen (1976).
The introduction of the two-step isolation procedure using collagenase by Seglen (1976) signified an important progress in primary liver cell isolation.
The isolation width for the ions from the samples of unmodified proteins and fractions of mono- and bis-nitrated lysozyme was 20−50 Th (single step isolation).
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