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This instrumental reduction of U is advantageous in cases that require rapid Pu analyses as it simplifies the chemistry of Pu/U separation and other steps in the sample processing.
According to this formula, detecting a signal produced by an analyte in its standard form leads to the instrumental detection limit, without considering any steps in the sample treatment.
For the chip design, a modular approach was followed allowing the optimization of individual steps in the sample processing process.
The 3'/5' signal ratio from these probe sets is informative about the reverse transcription and in vitro transcription steps in the sample preparation.
Following harvesting, the remaining sample steps in the sample preparation process closely matched the steps of the two-proteome sample (Supporting Information).
During the long-range PCR and PCR steps in the sample preparation steps for the Illumina platform, indels can occur in microsatellite loci, leading to less or more repeats.
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The selection of target species according to well-defined criteria as well as the selection of target compartments are crucial steps in the sampling process.
Positioning of embryos is an important step in the sample preparation for capturing video.
As an additional step in the sample preparation, special primers were ligated onto both ends of each fragment, identifying the fragments.
The in-solution approach tends to be the simplest in terms of sample handling and speed, but the digestion step is still the most time-consuming step in the sample preparation workflow.
Detailed information on patient attrition at each step in the sample selection process has been provided in Tables 1 and 2, and patient demographics are shown in Tables 3 and 4.
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