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It appears that individual serpins may inhibit proteinases in different proteolytic cascades; while particular physiological responses might be modulated by serpins targeting different steps in the same proteinase cascade.
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In these experiments the same proteinase activity was used.
An αS fibrillar sample with an estimated concentration of around 30 μM was incubated with the same proteinase K concentrations in the same way as for the monomeric sample, but in this case a serine-protease inhibitors cocktail (PMSF, Sigma-Aldrich ) was added in 1200 1 ratio with respect to the proteinase K concentration to stop the degradation reaction.
The same proteinase-K stock was used through-out the experimental period.
HistoricPlacesLA is a step in the same direction.
Step in the same river twice?
Step 1, Step 2, Step 4, Step 5, and Step 6 are the same as those in Algorithm 3.
But the general steps are the same.
The steps are the same each time.
The steps are the same.
All other steps are the same.
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