Sentence examples for steps in the same buffer from inspiring English sources

After two washing steps in the same buffer, samples were postfixed with 1% (w/v) OsO4 in cacodylate buffer for 2 h at 4°C, washed again, dehydrated and embedded in an Epon-Araldite mixture according to standard protocols.

After a 4 CV washing step in the same buffer, the recombinant protein was eluted applying a linear imidazole gradient (25 250 mM over 18 CV).

After a washing step (4 CV) in the same buffer, the samples were eluted applying a linear imidazole gradient (25 250 mM over 18 CV).

The suspended membrane vesicles were layered over a 21-ml five steps gradient of sucrose dissolved in the same buffer (3 ml of 50%, 9 ml of 45%, 3 ml of 40%, 3 ml of 35% and 3 ml of 30% from bottom to top) in a bottle assembly polycarbonate tube (part no. 355618, Beckman Coulter) and centrifuged at 47 000 r.p.m. for 2 h at 4̊C with the type 50.2 Ti rotor (Beckman Coulter).

Gels were then washed with 10 volumes of buffer A before being eluted step by step with increasing amounts of NaCl dissolved in the same buffer: 10 volumes of 0.2 M NaCl for step 1; 10 volumes of 0.3 M NaCl for step 2; 10 volumes of 0.4 M NaCl for step 3, and 3 volumes of 0.8 M NaCl for step 4.

Nucleation intermediates were visualized using 25 µM bovine tubulin in BRB80, 1 mM GMPCPP, 4 mM MgCl2 and 10% DMSO that was subsequently diluted 10× in BRB80, MgCl2 and DMSO or using 2.5 µM porcine tubulin in the same buffer without dilution steps (figure 6B).

Primary antibodies were diluted in the same buffer while washing steps were carried out with TBST.

In the first run, UspA1 527 665) at 4 μM in 50 mM Tris HCl, pH 8.0, 150 mM NaCl, was titrated with a 47 μM solution of N-CEACAM1 in the same buffer in 25 steps of 10 μl; these concentrations were 5 and 100 μM, respectively, in the second run.

The column was washed with 100 ml of 100 mM NaCl in 5 mM sodium phosphate pH6.5 and bound proteins were eluted, collecting 10 ml fractions, in a linear gradient of 100-300 mM NaCl in the same buffer, all column steps were performed at 4ºC with the aid of gravity.

In order to reduce the background, plates were soaked for 10 to 15 minutes in the same buffer after the last washing step.

PepP was eluted in a step in which the imidazole concentration was increased to 500 mM (after ~ 40 min) in the same buffer.