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Technical advances in simultaneously manipulating multiple steps in plant metabolic pathways include the use of transcription factors, such as MYB12.
The first steps in plant xanthophyll biosynthesis are the hydroxylation of α- and β-carotene.
To improve those traits, researchers must have a keen molecular understanding of the mechanisms that modulate those steps in plant growth.
CYP450 proteins are the largest family of plant proteins and catalyze most of the oxidation steps in plant secondary metabolism [ 25, 26].
miR160 and miR164 targeted Auxin response factor (ARF) and NAC transcription factor (NAC), respectively (Additional file 11), which control key steps in plant development.
Cytochrome P450 proteins are the largest family of plant proteins and catalyze most of the oxidation steps in plant secondary metabolism [ 27, 28].
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The total number of such steps in plants, animals, fungi, and protists (see below Hypotheses of origins) is about 11.
By unraveling the basis of carotenoid biosynthesis it has become possible to modulate the key metabolic steps in plants and thus increase the nutritional value of staple crops, such as rice (Oryza sativa), maize (Zea mays) and potato (Solanum tuberosum).
Designing effective synthetic genes of interest is a fundamental step in plant synthetic biology for biomass.
Until recently the assembly of amplified or cloned sequences into large and complex designs was a limiting step in plant synthetic biology and biotechnology.
GDP-D-mannose pyrophosphorylase (GMP, EC 2.7.7.22) catalyzes the synthesis of GDP-D-mannose and represents the first committed step in plant ascorbic acid biosynthesis.
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